Killifish metallothionein messenger RNA expression following temperature perturbation and cadmium exposure
نویسندگان
چکیده
منابع مشابه
Metallothionein-like protein in the least killifish Heterandria formosa and its role in cadmium resistance.
Metallothioneins (MTs) are a class of low molecular weight, cysteine-rich, metal-binding proteins ubiquitous in animals. They function in metal regulation and detoxification. An MT-like protein was separated by gel-permeation high-performance liquid chromatography (HPLC) in the least killifish Heterandria formosa exposed to 6 mg/L of Cd for 26 h. Western blot analysis showed that this protein c...
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Efficient implementation of an environmental biomarker requires multi-annual comparability over a wide geographical range. The present study improved the comparability of a quantitative competitive metallothionein (MT) enzyme-linked-immuno-sorbent-assay (ELISA) in the sentinel fish Lithognathus mormyrus by introducing to the assay recombinant MT and beta-actin standards. Commercial antibodies f...
متن کاملLymphocyte metallothionein-mRNA as a sensitive biomarker of cadmium exposure.
Human peripheral blood lymphocytes (PBLs) produce metallothioneins (MTs) in response to a variety of heavy metal ions. MTs could therefore be a candidate for a marker that represents the biological effect of heavy metals. Since it is practically difficult to measure MT protein levels in PBLs, we examined if MT-mRNA could serve as a biomarker of heavy metal exposure. It is difficult to obtain RN...
متن کاملTime-dependent Changes of Cadmium and Metallothionein after Short-term Exposure to Cadmium in Rats
The time-dependent changes in cadmium (Cd) concentration were studied in Female Sprague-Dawley (SD) rats during and after Cd exposure via drinking water (10 and 50 ppm) for 30 days. The cadmium concentration in muscle, liver, kidney, blood plasma, and urine, and the metallothionein concentration in blood plasma were determined every 10 days during exposure and every 7 days after exposure for 3 ...
متن کاملChallenging the Metallothionein (MT) Gene of Biomphalaria glabrata: Unexpected Response Patterns Due to Cadmium Exposure and Temperature Stress
Metallothioneins (MTs) are low-molecular-mass, cysteine-rich, metal binding proteins. In most animal species, they are involved in metal homeostasis and detoxification, and provide protection from oxidative stress. Gastropod MTs are highly diversified, exhibiting unique features and adaptations like metal specificity and multiplications of their metal binding domains. Here, we show that the MT ...
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ژورنال
عنوان ژورنال: Cell Stress & Chaperones
سال: 2001
ISSN: 1355-8145,1466-1268
DOI: 10.1379/1466-1268(2001)006<0351:kmmref>2.0.co;2